CGRP antibody

Catalog No. ABIN361373

The Mouse Monoclonal anti-CGRP antibody has been validated for ICC. It is suitable to detect CGRP in samples from Rat.

Quick Overview for CGRP antibody (ABIN361373)

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CGRP antibody is un-conjugated

Application: Immunocytochemistry (ICC)

Clone: 4901

Product Details anti-CGRP Antibody

Specificity: Specific for Calcitonin Gene Related Peptide. Also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP). Suggested positive controls are dorsal root ganglia, pancreas and gut. Notes: This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue. It is expected that the antibody will react with most mammals.

Cross-Reactivity: Human, Rat (Rattus)

Predicted Reactivity: most mammals

Purification: Protein G purified culture supernatant

Immunogen: Purified rat alpha-CGRP

Isotype: IgG2a

Application Details

Application Notes: Recommended Dilution: ICC: 1:50-1:100 Quality Control: ICC performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for CGRP

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Alternative Name: CALCA

Background: Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in immunoneutralization (Wong et al., 1993)

Gene ID: 24241

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour