GABRA3 antibody (N-Term)

Catalog No. ABIN361437

This anti-GABRA3 antibody is a Rabbit Polyclonal antibody detecting GABRA3 in WB and IHC. Suitable for Rat.

Quick Overview for GABRA3 antibody (N-Term) (ABIN361437)

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABRA3 Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~51k a3-subunit of the GABAA receptor in Western blots. Labeling is absent in a3-subunit knockout animals.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, human, non-human primates, Zebrafish

Purification: Antigen Affinity Purified

Immunogen: Synthetic peptide from the N-terminal region of the alpha 3 subunit

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for GABRA3

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Alternative Name: GABRA3

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, fourbetas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and gamma-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, P ltl et al., 2003). Anti-GABAA-Receptor, 3 -Subunit Western blot of mouse brain lysates from wild type (Control) and a3-knockout (aK/O) animals showing specific immunolabeling of the ~51k a3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a3-knockout animals.

Molecular Weight: '51 kDa

Gene ID: 24947

UniProt: P20236