GAP43 antibody (pSer41)

Catalog No. ABIN361475

There is 1 publication for this product available. The Rabbit Polyclonal anti-GAP43 antibody has been validated for WB and ELISA. It is suitable to detect GAP43 in samples from Rat.

Quick Overview for GAP43 antibody (pSer41) (ABIN361475)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GAP43 Antibody

Binding Specificity: pSer41

Specificity: Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, finch, human, non-human primates, Xenopus, zebra fish

Characteristics: Our Gap-43 (Ser41) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Gap-43 (Ser41) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-GAP43 antibody (ABIN361475)

Yi, Kim, Park, Yu, Kim: "The effect of systemic injection of cyclosporin A on the phosphorylation of the PKC substrates MARCKS and GAP43 in the rat hippocampus." in: Neuroscience letters, Vol. 497, Issue 1, pp. 17-21, (2011) (PubMed).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background:

Synonyms: Axonal membrane protein GAP 43 antibody, Axonal membrane protein GAP-43 antibody, B 50 antibody, Calmodulin binding protein P 57 antibody, F1 antibody, GAP 43 antibody, GAP-43 antibody, GAP43 antibody, Growth Associated Protein 43 antibody, Growth-associated protein 43 antibody, Nerve Growth Related Peptide antibody, Nerve growth related peptide GAP43 antibody, NEUM_HUMAN antibody, Neural phosphoprotein B 50 antibody, Neural phosphoprotein B-50 antibody, Neuromodulin antibody, Neuron growth associated protein 43 antibody, PP46 antibody, Protein F1 antibody, QtrA-11580 antibody, QtrA-13071 antibody

Description Logic: Our Gap-43 (Ser41) rabbit polyclonal phosphospecific primary antibody is produced . It detects Gap-43 (Ser41) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: GAP43

Molecular Weight: 50 kDa

Gene ID: 29423

UniProt: P07936