SYT1 antibody (pSer309)

Catalog No. ABIN361497

This anti-SYT1 antibody is a Rabbit Polyclonal antibody detecting SYT1 in WB and IHC. Suitable for Rat. This Primary Antibody has been cited in 5+ publications.

Quick Overview for SYT1 antibody (pSer309) (ABIN361497)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYT1 Antibody

Binding Specificity: pSer309

Specificity: Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-SYT1 antibody (ABIN361497)

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Poskanzer, Marek, Sweeney, Davis: "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo." in: Nature, Vol. 426, Issue 6966, pp. 559-63, (2003) (PubMed).

Wang, Lu, Bai, Chang, Martin, Chapman, Jackson: "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion." in: Nature, Vol. 424, Issue 6951, pp. 943-7, (2003) (PubMed).

Fernández-Chacón, Königstorfer, Gerber, García, Matos, Stevens, Brose, Rizo, Rosenmund, Südhof: "Synaptotagmin I functions as a calcium regulator of release probability." in: Nature, Vol. 410, Issue 6824, pp. 41-9, (2001) (PubMed).

Hilfiker, Pieribone, Nordstedt, Greengard, Czernik: "Regulation of synaptotagmin I phosphorylation by multiple protein kinases." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 921-32, (1999) (PubMed).

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: SYT1

Molecular Weight: 60/62 kDa

Gene ID: 25716

UniProt: P21707

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport