DNAJB1 antibody

Catalog No. ABIN361662

The Mouse Monoclonal anti-DNAJB1 antibody has been validated for WB, ELISA and IP. It is suitable to detect DNAJB1 in samples from Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for DNAJB1 antibody (ABIN361662)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNAJB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 2A7-H6

Product Details anti-DNAJB1 Antibody

Specificity: Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.

Cross-Reactivity: Saccharomyces cerevisiae

Purification: Protein G Purified

Immunogen: Full length protein HSP40 (YDJ1)

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-DNAJB1 antibody (ABIN361662)

Knighton, Nitika, Waller, Strom, Wolfgeher, Reitzel, Truman: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." in: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).

Sluder, Nitika, Knighton, Truman: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." in: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: HSP40

Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

Gene ID: 855661

NCBI Accession: NP_014335

UniProt: P25491