AHSA1 antibody

Catalog No. ABIN361854

The Rabbit Polyclonal anti-AHSA1 antibody has been validated for WB, ELISA, IHC, IP, IF and ICC. It is suitable to detect AHSA1 in samples from Mouse. There is 1 publication available.

Quick Overview for AHSA1 antibody (ABIN361854)

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AHSA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-AHSA1 Antibody

Specificity: Detects ~ 38 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Recombinant Full Length Mouse Aha1 Protein

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:60)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μl/ml of ABIN361853 was sufficient for detection of Aha1 in 10 μg of mixed human cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-AHSA1 antibody (ABIN361854)

Echeverría, Bernthaler, Dupuis, Mayer, Picard: "An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine." in: PLoS ONE, Vol. 6, Issue 10, pp. e26044, (2011) (PubMed).

Target Details for AHSA1

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Alternative Name: AHA1

Background: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Gene ID: 217737

NCBI Accession: NP_666148

UniProt: Q8BK64