ICAM1 antibody (Intercellular Adhesion Molecule 1) (pTyr512)

Catalog No. ABIN362032

Product Details anti-ICAM1 Antibody

Target: ICAM1

Binding Specificity: pTyr512

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of ICAM-1 only when phosphorylated at tyrosine 512.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr512 (K-K-Y (p) -R-L) derived from Human ICAM-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000  

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for ICAM1

Target: ICAM1

Alternative Name: ICAM-1 (ICAM1 Antibody Abstract)

Synonyms: BB2, CD54, P3.58, Icam-1, Ly-47, MALA-2, ICAM, ICAM-1, ICAM1, intercellular adhesion molecule 1, intercellular adhesion molecule-1, ICAM1, Icam1, ICAM-1

Background: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin a-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Molecular Weight: 92 kDa

NCBI Accession: NP_000192

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling