HDAC5 antibody (AA 496-500)

Catalog No. ABIN362746

Cited in 2+ publications. The Rabbit Polyclonal anti-HDAC5 antibody (ABIN362746) specifically detects HDAC5 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HDAC5 antibody (AA 496-500) (ABIN362746)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 496-500

Specificity: The antibody detects endogenous level of total HDAC5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 496-500 (T-Q-S-S-P) derived from Human HDAC5, 7. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-HDAC5 antibody (ABIN362746)

Wang, Ha, Jhun, Wong, Jain, Jin: "Fluid shear stress stimulates phosphorylation-dependent nuclear export of HDAC5 and mediates expression of KLF2 and eNOS." in: Blood, Vol. 115, Issue 14, pp. 2971-9, (2010) (PubMed).

Pang, Yan, Natarajan, Cavet, Massett, Yin, Berk: "GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 5, pp. 892-8, (2008) (PubMed).

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 124 kDa

NCBI Accession: NP_001015

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process