TNNI3 antibody (AA 41-49)

Catalog No. ABIN363301

This Mouse Monoclonal antibody specifically detects TNNI3 in WB, IHC, ELISA, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TNNI3 antibody (AA 41-49) (ABIN363301)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 19C7

Product Details anti-TNNI3 Antibody

Binding Specificity: AA 41-49

Specificity: Reacts with protein from Human, Mouse, Rat, Bovine, Canine, Feline, Fish, Goat, Porcine and Rabbit

Cross-Reactivity (Details): Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Purification: Purified (protein A)

Immunogen: Native troponin complex, (epitope 41-49 aa)

Isotype: IgG2b

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB1:100-1:2000 IHC1:10-1:500 IP1:10-1:500 Sandwich ELISA1:100-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 3.2 mg/mL

Buffer: PBS, ( pH 7.4), Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Troponin I, Cardiac Muscle

Background: Belongs to the troponin I family. Defects in TNNI3 are the cause of familial hypertrophic cardiomyopathy type 7 (CMH7), familial restrictive cardiomyopathy type 1 (RCM1), cardiomyopathy dilated type 2A (CMD2A) and cardiomyopathy dilated type 1FF (CMD1FF).

UniProt: P19429