Calmodulin 1 antibody (AA 1-149)

Catalog No. ABIN364349

The Mouse Monoclonal anti-Calmodulin 1 antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect Calmodulin 1 in samples from Human.

Quick Overview for Calmodulin 1 antibody (AA 1-149) (ABIN364349)

Target: Calmodulin 1 (Calm1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calmodulin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: J4D8

Product Details anti-Calmodulin 1 Antibody

Binding Specificity: AA 1-149

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human Calmodulin (1-149aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Calmodulin 1

Target: Calmodulin 1 (Calm1)

Alternative Name: Calmodulin

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling