TCR gamma/delta antibody

Catalog No. ABIN371050

This Hamster Monoclonal antibody specifically detects TCR gamma/delta in FACS, IP, IHC (fro) and Func. It exhibits reactivity toward Mouse.

Quick Overview for TCR gamma/delta antibody (ABIN371050)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: UC7-13D5

Product Details anti-TCR gamma/delta Antibody

Characteristics: Synonyms: TCRG, TCRD, T-Cell Receptor gamma, T-Cell Receptor delta, T-Cell Receptor gamma delta

Endotoxin Level: Low Endotoxin

Isotype: IgG

Application Details

Application Notes: Flow Cytometry. (Ref.4-7)Immunoprecipitation. (Ref.5)Immunohistochemistry on Frozen Sections. (Ref.8)In vitro Activation of gamma-delta TCR-bearing cells. (Ref.5)In vivo Depletion of gamma-delta TCR-expressing T cells. (Ref.5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing no preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquous) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: T Cell Receptor / TCR gamma/delta

Background: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. (Ref.1) It is expressed during thymopoiesis and on the small subpopulation of gamma/delta TCR expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma/delta+ and have limited receptor diversity. (Ref.1,2) The TCR gamma/delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation. (Ref.3)Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG