MHC Class II antibody

Catalog No. ABIN371399

The Mouse Monoclonal anti-MHC Class II antibody has been validated for FACS, WB, IHC (fro) and IP. It is suitable to detect MHC Class II in samples from Chicken.

Quick Overview for MHC Class II antibody (ABIN371399)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: 2G11

Product Details anti-MHC Class II Antibody

Characteristics: Synonyms: B-LA, Major Histocompatibility Complex Class II

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.2-6)Immunoprecipitation. (Ref.7)Immunohistochemistry. (Ref.6)Immunoblotting. (Ref.6)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II

Background: MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.Synonyms: HLA Class II, Human Leukocyte antigen class II, MHC Class 2, Major histocompatibility complex class II