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BU-1A / B6.1 antibody (FITC)

This Mouse Monoclonal antibody specifically detects BU-1A / B6.1 in FACS. It exhibits reactivity toward Chicken.
Catalog No. ABIN371410

Quick Overview for BU-1A / B6.1 antibody (FITC) (ABIN371410)

Target

BU-1A / B6.1

Reactivity

  • 13
  • 2
Chicken

Host

  • 13
Mouse

Clonality

  • 13
Monoclonal

Conjugate

  • 4
  • 4
  • 3
  • 2
This BU-1A / B6.1 antibody is conjugated to FITC

Application

  • 13
  • 8
  • 7
  • 5
Flow Cytometry (FACS)

Clone

21-1A4
  • Specificity

    This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence.

    Characteristics

    Synonyms: B-Cell Marker, BU-1 Allele A

    Immunogen

    Bursal cells from one-day-old H. B15 (Bu-1a/b) strain chickens.

    Isotype

    IgG1
  • Application Notes

    Flow Cytometry: < / = 1 μg/10^6 cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    PBS containing 0.09 % Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    Expiry Date

    12 months
  • Target

    BU-1A / B6.1

    Background

    Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.Synonyms: B-Cell Marker, BU-1 Allele A

    Gene ID

    396098

    UniProt

    Q90746
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