CGRP antibody

Catalog No. ABIN371823

This anti-CGRP antibody is a Mouse Monoclonal antibody detecting CGRP in IF and IHC (fro). Suitable for Human, Rat and Mammalian.

Quick Overview for CGRP antibody (ABIN371823)

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivity: Human, Rat, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This CGRP antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4901

Product Details anti-CGRP Antibody

Specificity: This antibody is specific for Calcitonin Gene Related Peptide. Also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP). Suggested Positive Controls: Dorsal root ganglia, pancreas and gut.

Cross-Reactivity (Details): Species reactivity (expected):most Mammals.
Species reactivity (tested):Human and Rat.

Characteristics: Synonyms: Calcitonin gene-related peptide I, Alpha-type CGRP, CALC1, CGRP-I

Purification: Protein G Chromatography.

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry: 1/50-1/100. Immunofluorescence and Immunohistochemistry: This antibody is an excellent tool forImmunohistochemistry and for multiple labeling immunofluorescence of peripheral tissuelike stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that areinnervated by primary afferent neurons as well as central nervous tissue. Suggested Positive Controls: Dorsal root ganglia, pancreas and gut. Not recommended for Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CGRP

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Alternative Name: CGRP / CALCA

Background: Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in Immunoneutralization (Wong et al., 1993)Synonyms: Alpha-type CGRP, CALC1, CGRP-I, Calcitonin gene-related peptide I

Gene ID: 796

UniProt: P06881

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour