XPO1 antibody (AA 390-408)

Catalog No. ABIN372248

The Rabbit Polyclonal anti-XPO1 antibody has been validated for WB and IHC (p). It is suitable to detect XPO1 in samples from Human, Mouse, Rat, Cow, Dog and Chimpanzee.

Quick Overview for XPO1 antibody (AA 390-408) (ABIN372248)

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XPO1 Antibody

Binding Specificity: AA 390-408

Sequence: ASPLLSGSQH FDVPPRRQL

Specificity: This antibody recognizes CRM1 Protein (XPO1).

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Chimpanzee, Dog, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to the amino acids 390-408 of human Exportin-1 protein.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 10 μg/mL. Western Blot: 1-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative and 0.2 % Gelatin as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: XPO1 / Exportin-1

Background: CRM1 (chromosome region maintenance 1 protein homolog) mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1.Synonyms: CRM1, Chromosome region maintenance 1 protein homolog, Exp1

Gene ID: 7514

NCBI Accession: NP_003391

UniProt: O14980

Pathways: M Phase