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Connexin 43/GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (pSer368) Primary Antibody

GJA1 Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN372603
$568.70
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0.1 mL
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  • Target
    Connexin 43/GJA1 (GJA1)
    Binding Specificity
    • 25
    • 16
    • 16
    • 13
    • 8
    • 8
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer368
    Reactivity
    • 146
    • 100
    • 96
    • 36
    • 13
    • 12
    • 10
    • 10
    • 9
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio)
    Host
    • 141
    • 10
    • 10
    Rabbit
    Clonality
    • 149
    • 11
    Polyclonal
    Conjugate
    • 95
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Connexin 43/GJA1 antibody is un-conjugated
    Application
    • 111
    • 60
    • 58
    • 32
    • 21
    • 19
    • 13
    • 13
    • 11
    • 10
    • 9
    • 6
    • 2
    • 2
    • 1
    Western Blotting (WB)
    Specificity
    Specific for the ~43k Connexin-43 protein phosphorylated at Ser368. Immunolabeling is blocked by λ-phosphatase treatment.
    Cross-Reactivity (Details)
    Species reactivity (expected):Bovine, Canine, Chicken, Guinea Pig, Human, Mouse and Zebrafish.
    Species reactivity (tested):Rat.
    Purification
    Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
    Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat Connexin-43.
    Isotype
    IgG
  • Application Notes
    Western Blot: 1/1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted (in aliquots) at-20 °C.
  • Target
    Connexin 43/GJA1 (GJA1)
    Alternative Name
    GJA1 / Cx43 (GJA1 Antibody Abstract)
    Synonyms
    AVSD3, CX43, DFNB38, GJAL, HLHS1, HSS, ODDD, Cx43, AU042049, AW546267, Cnx43, Cx43alpha1, Gja-1, Npm1, connexin43, cx43a1, gja1, zfCx43.3, connexin 43, gja1-A, cx43, gjal, oddd, dfnb38, gap junction protein alpha 1, gap junction protein, alpha 1, connexin 43, gap junction protein alpha 1 L homeolog, CONNEXIN 43 protein, GJA1, Gja1, cx43, gja1.L, gja1, CONNEXIN 43
    Background
    Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
    Gene ID
    2697
    NCBI Accession
    NP_000156
    UniProt
    P17302
    Pathways
    MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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