GAP43 antibody (pSer41)

Catalog No. ABIN372649

This Rabbit Polyclonal antibody specifically detects GAP43 in WB and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Chicken, Cow, Zebrafish (Danio rerio), Dog, Xenopus laevis, Finch and Non-Human Primate.

Quick Overview for GAP43 antibody (pSer41) (ABIN372649)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Zebrafish (Danio rerio), Dog, Xenopus laevis, Finch, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAP43 Antibody

Binding Specificity: pSer41

Specificity: Specific for the ~50k Gap-43 protein phosphorylated at Ser41 in Western Blots of Rat brain lysate. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with lambda-Ptase.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Chicken, Finch, Human, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemitsry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46

Gene ID: 29423

NCBI Accession: NP_058891

UniProt: P07936