P4HB antibody

Catalog No. ABIN372745

This Rabbit Polyclonal antibody specifically detects P4HB in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for P4HB antibody (ABIN372745)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P4HB Antibody

Specificity: Recognizes endogenous levels of total P4HB.

Cross-Reactivity (Details): Species reactivity (expected):Monkey, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide conjugated to KLH derived from the sequence around Proline 329 of Human P4HB.

Isotype: IgG

Application Details

Application Notes: Western Blot. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Sodium HEPES, pH 7.5, 150 mM Sodium Chloride, 0.1 mg/mL BSA, 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI / P4HB

Background: P4HB, Prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.Synonyms: Cellular thyroid hormone-binding protein, ER marker, ERBA2L, P4HB, PDI, PDIA1, PO4DB, Prolyl 4-hydroxylase subunit beta, Protein Disulfide Isomerase, Protein disulfide-isomerase, p55

Molecular Weight: 57 kDa.

Gene ID: 5034

NCBI Accession: NP_000909

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism