Cathepsin K antibody

Catalog No. ABIN372829

This Rabbit Polyclonal antibody specifically detects Cathepsin K in WB, IP and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Pig, Rabbit and Chicken.

Quick Overview for Cathepsin K antibody (ABIN372829)

Target: Cathepsin K (CTSK)

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin K antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cathepsin K Antibody

Specificity: The antibody detects proform (43 kDa) and the mature form (29 kDa) of Cathepsin K.

Cross-Reactivity (Details): Species reactivity (expected):Chicken, Human, Mouse, Porcine, Rabbit.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide surrounding amino acid 321 of Rat Cathepsin K .

Isotype: IgG

Application Details

Application Notes: Western blot: 0.5-4 μg/mL. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Cathepsin K

Target: Cathepsin K (CTSK)

Alternative Name: Cathepsin K

Background: Cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin K expression is highest in bone, cartilage, and skeletal muscle. Cathepson K is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in the gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature.Synonyms: CTSK, CTSO, CTSO2, Cathepsin O, Cathepsin O2, Cathepsin X

Gene ID: 1513

NCBI Accession: NP_000387

UniProt: P43235

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades