PPP2R3A antibody (Internal Region)

Catalog No. ABIN372864

Product Details anti-PPP2R3A Antibody

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to Protein Phosphatase Pp2A, 130 Kd Regulatory Subunit (PPP2R3A).

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-QKDVENDGPEPSD) from an internal region of Human PPP2R3A

Isotype: IgG

Application Details

Application Notes: ELISA: 1/16000. Western blot: 1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This PPP2R3A antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris Saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for PPP2R3A

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Alternative Name: PPP2R3A / PPP2R3 (PPP2R3A Products)

Synonyms: PPP2R3 antibody, PR130 antibody, PR72 antibody, 3222402P14Rik antibody, A730042E07 antibody, PPP2R3A antibody, Ppp2r3a antibody, protein phosphatase 2 regulatory subunit B''alpha antibody, protein phosphatase 2, regulatory subunit B'', alpha antibody, protein phosphatase 2 regulatory subunit B, alpha S homeolog antibody, serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha antibody, PPP2R3A antibody, Ppp2r3a antibody, ppp2r3a.S antibody, LOC100088744 antibody, LOC100478108 antibody, ppp2r3a antibody, LOC100547843 antibody, LOC100729010 antibody

Background: Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.Synonyms: B72/B130 isoforms, PP2A subunit B B''-PR72/PR130, PP2A subunit B PR72/PR130 isoforms, Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B, Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, phosphatase 2A regulatory subunit B'' alpha

Gene ID: 5523

NCBI Accession: NP_001177376

UniProt: Q06190

Pathways: PI3K-Akt Signaling