PPP2R3A antibody (Internal Region)

Catalog No. ABIN238630

This Goat Polyclonal antibody specifically detects PPP2R3A in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PPP2R3A antibody (Internal Region) (ABIN238630)

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PPP2R3A Antibody

Binding Specificity: Internal Region

Purpose: PPP2R3A

Sequence: QKDVENDGPE PSD

Specificity: This antibody is expected to recognise both reported isoforms (NP_002709.2, NP_871626.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QKDVENDGPEPSD, from the internal region of the protein sequence according to NP_002709.2, NP_871626.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2R3A

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Alternative Name: PPP2R3A

Background: PPP2R3A, protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha , PPP2R3, PR130, PR72, PP2A, subunit B, B''-PR72/PR130, PP2A, subunit B, B72/B130 isoforms, PP2A, subunit B, R3 isoform, Serine/threonine protein phosphatase 2A, 72/130 kDa regul

Gene ID: 5523

NCBI Accession: NP_002709, NP_871626

Pathways: PI3K-Akt Signaling