DRAM antibody (C-Term)

Catalog No. ABIN372924

This anti-DRAM antibody is a Rabbit Polyclonal antibody detecting DRAM in WB, IHC (p), EIA and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for DRAM antibody (C-Term) (ABIN372924)

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DRAM Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes Damage-regulated Autophagy Modulator (DRAM).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated corresponding to a 16 amino acids peptide from near the carboxy terminus of Human DRAM.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry on Frozen Sections: 2.5 μg/mL. Immunohistochemistry on Paraffin Sections: 3 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DRAM

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Alternative Name: DRAM1

Background: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.Synonyms: DNA damage regulated autophagy modulator protein 1, DRAM, DRAM-1, FLJ11259

Gene ID: 55332

NCBI Accession: NP_060840

UniProt: Q8N682