STRADB antibody (C-Term)

Catalog No. ABIN374305

This Goat Polyclonal antibody specifically detects STRADB in WB and IHC (p). It exhibits reactivity toward Human, Cow and Dog.

Quick Overview for STRADB antibody (C-Term) (ABIN374305)

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Reactivity: Human, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This STRADB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STRADB Antibody

Binding Specificity: C-Term

Specificity: This antibody detects STRAD beta / ILPIP at C-term. This is expected to recognise both human isoforms: ILPIP-alpha and ILPIP-beta.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human

Purification: Affinity chromatography

Immunogen: Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence Genename: STRADB

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL. Approx 35 kDa band observed in Human Heart, Liver and Musclelysates (calculated MW of 47.5 kDa according to NP_061041.2 (alpha isoform) and 31.5 kDaaccording to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIPa band of approx.45 kDa is observed. This band is not observed in the non-transfectedHEK293. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STRADB

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Alternative Name: STRAD beta / ILPIP

Background: Synonyms: ALS2CR2, CALS-21, PRO1038, Pseudokinase ALS2CR2, STE20-related kinase adapter protein beta, STRADB

Gene ID: 55437

NCBI Accession: NP_061041

UniProt: Q9C0K7

Pathways: AMPK Signaling