FBXO43 antibody (C-Term)

Catalog No. ABIN374351

The Goat Polyclonal anti-FBXO43 antibody has been validated for WB, IF, EIA and FACS. It is suitable to detect FBXO43 in samples from Human, Mouse, Rat, Cow, Dog and Pig.

Quick Overview for FBXO43 antibody (C-Term) (ABIN374351)

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO43 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Product Details anti-FBXO43 Antibody

Binding Specificity: C-Term

Sequence: C-PGSAQSKRNL KRL

Specificity: Recognizes EMI2.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FBXO43

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Alternative Name: FBXO43

Background: Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B

Gene ID: 286151

NCBI Accession: NP_001025031

UniProt: Q4G163