Glucose-6-Phosphate Dehydrogenase antibody (Internal Region)

Catalog No. ABIN374802

This anti-Glucose-6-Phosphate Dehydrogenase antibody is a Goat Polyclonal antibody detecting Glucose-6-Phosphate Dehydrogenase in WB. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for Glucose-6-Phosphate Dehydrogenase antibody (Internal Region) (ABIN374802)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Binding Specificity: Internal Region

Sequence: C-KPASTNSDDV RDEK

Specificity: This antibody detetcs G6PD. It is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1 Genename: G6PD

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Placenta lysates(calculated MW of 62.0 kDa according to NP_000393.4 and 59.0 kDa according toNP_001035810.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD

Background: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase

Gene ID: 2539, 9606

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones