FTL antibody (C-Term, Light Chain)

Catalog No. ABIN375068

There is 1 publication for this product available. The Goat Polyclonal anti-FTL antibody is suitable to detect FTL in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for FTL antibody (C-Term, Light Chain) (ABIN375068)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-FTL Antibody

Binding Specificity: C-Term, Light Chain

Sequence: C-GEYLFERLTL KHD

Specificity: This antibody recognizes FTL.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide (C-GEYLFERLTLKHD) from the C-Terminus of Human FTL (NP_000137.2). Genename: FTL

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-FTL antibody (ABIN375068)

Sammarco, Ditch, Banerjee, Grabczyk: "Ferritin L and H subunits are differentially regulated on a post-transcriptional level." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4578-87, (2008) (PubMed).

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin Light Chain (FTL)

Background: Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: Ferritin L subunit

Molecular Weight: 20.0 kDa (NP_000137.2).

Gene ID: 2512

NCBI Accession: NP_000137

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis