CD5 antibody (APC)

Catalog No. ABIN375394

This Rat Monoclonal antibody specifically detects CD5 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD5 antibody (APC) (ABIN375394)

Target: CD5

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: B19-1

Product Details anti-CD5 Antibody (APC)

Specificity: Mouse CD5, Mr 67 kDa

Characteristics: Rat Anti-Mouse CD5-APC

Purification: Purified

Immunogen: Unknown

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , WB - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5 + B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5 + B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.