CD40 antibody (Biotin)

Catalog No. ABIN375940

This Rat Monoclonal antibody specifically detects CD40 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD40 antibody (Biotin) (ABIN375940)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 1C10

Product Details anti-CD40 Antibody (Biotin)

Specificity: Mouse CD40, Mr 45-50 kDa

Characteristics: Rat anti-Mouse CD40-BIOT

Purification: Purified

Immunogen: sCD40

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , Activ - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.5 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro induction of proliferation of mature B lymphocyte

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs. CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM + IgD lo B220 lo ) express intermediate levels of CD40, whereas mature B cells (IgM + IgD hi B220 hi ) express high levels. CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints