Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Biotin)

Catalog No. ABIN375969

Quick Overview for Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN375969)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Specificity: Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and/or flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with normal rat IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on mouse IgA covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
<= 1 µg/10^6 cells or 1:5,000-1:20,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C

References

van den Borne, Rygiel, Hoogendoorn, Westerlaken, Boon, Quax, Pasterkamp, Hoefer, Meyaard: "The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx." in: PLoS ONE, Vol. 9, Issue 6, pp. e98820, (2014) (PubMed).

Target Details

Target: IgG

Target Type: Antibody