Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Biotin)

Catalog No. ABIN375993

Quick Overview for Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN375993)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Rabbit

Specificity: Reacts with the heavy and light chains of human IgG as demonstrated by ELISA.

Characteristics: Source: Pooled antisera from rabbits hyperimmunized with human IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA and PCFIA for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on canine IgG covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
<= 1 µg/10^6 cells or 1:5,000-1:20,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C

References

Aghamohammadi, Hosseini, Srikant, Wong, Poudineh: "Computational and Experimental Model to Study Immunobead-Based Assays in Microfluidic Mixing Platforms." in: Analytical chemistry, Vol. 94, Issue 4, pp. 2087-2098, (2022) (PubMed).

Target Details

Target: IgG

Target Type: Antibody