CD3 antibody

Catalog No. ABIN376394

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS, IP and IHC. It is suitable to detect CD3 in samples from Human.

Quick Overview for CD3 antibody (ABIN376394)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: UCHT1

Product Details anti-CD3 Antibody

Characteristics: The antibody detects: ε chain of the CD3/T cell antigen receptor complex
The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex.
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.

Purification: Purified

Endotoxin Level: Low Endotoxin

Isotype: IgG1

Application Details

Application Notes: Working Dilution:
<= 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Comment:

in vitro activation of T Cells

Sample Volume: 0,2 mL

Restrictions: For Research Use only

Handling

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80 % of normal peripheral blood lymphocytes and on 10-20 % of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway