Goat anti-Human IgG Antibody (PE)

Catalog No. ABIN376511

Quick Overview for Goat anti-Human IgG Antibody (PE) (ABIN376511)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Specificity: Reacts with the heavy chain of human IgG

Characteristics: Goat F(ab')2 Anti-Human IgG-PE

Purification: Purified

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , WB , Sep , Stim
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF555 conjugates 1:100 - 1:400 PE conjugate 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." in: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).

Cheng, Yang, Chen, Hsu, Jou, Hsieh, Tseng: "CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186780, (2017) (PubMed).

Trinklein, Pham, Schellenberger, Buelow, Boudreau, Choudhry, Clarke, Dang, Harris, Iyer, Jorgensen, Pratap, Rangaswamy, Ugamraj, Vafa, Wiita, van Schooten, Buelow, Force Aldred: "Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies." in: mAbs, Vol. 11, Issue 4, pp. 639-652 (PubMed).

Target Details

Target: IgG

Target Type: Antibody