CD3 antibody (SPRD)

Catalog No. ABIN376729

This Mouse Monoclonal antibody specifically detects CD3 in FACS. It exhibits reactivity toward Chicken and has been mentioned in 5+ publications.

Quick Overview for CD3 antibody (SPRD) (ABIN376729)

Target: CD3

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: CT-3

Product Details anti-CD3 Antibody (SPRD)

Brand: Spectral Red ™

Specificity: Chicken/Pigeon CD3, Mr 20, 19 & 17 kDa

Characteristics: Mouse Anti-Chicken CD3-SPRD

Purification: Purified

Immunogen: Chicken thymocytes and Ig- blood mononuclear cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.3 g/106 cells SPRD conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro depletion of CD3+ cells, In vivo activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 antibody (SPRD) (ABIN376729)

Peng, Yu, Liang, Chi, Li, Jiang, Fang, Cui, Lai, Zhou, Zhou: "The mitochondrial and death receptor pathways involved in the thymocytes apoptosis induced by aflatoxin B1." in: Oncotarget, Vol. 7, Issue 11, pp. 12222-34, (2017) (PubMed).

Peng, Zhang, Bai, Ding, Zeng, Yang, Fang, Chen: "Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn." in: International journal of environmental research and public health, Vol. 11, Issue 8, pp. 8567-80, (2015) (PubMed).

Chen, Peng, Fang, Cui, Zuo, Deng, Chen, Geng, Lai, Tang, Yang: "Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1." in: International journal of environmental research and public health, Vol. 11, Issue 2, pp. 1904-13, (2015) (PubMed).

Carballeda, Zoth, Gómez, Gravisaco, Berinstein: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, (2011) (PubMed).

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3 is a member of the T cell receptor-associated CD3 complex.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway