IKBKB
Reactivity: Human
ELISA, WB, IHC, IF
Host: Rabbit
Monoclonal
3E1
unconjugated
Recombinant Antibody
Application Notes
WB: 1:100~500. WB: 1:4000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date
6 months
Boisson, Laplantine, Dobbs, Cobat, Tarantino, Hazen, Lidov, Hopkins, Du, Belkadi, Chrabieh, Itan, Picard, Fournet, Eibel, Tsitsikov, Pai, Abel, Al-Herz, Casanova, Israel, Notarangelo: "Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia." in: The Journal of experimental medicine, Vol. 212, Issue 6, pp. 939-51, (2015) (PubMed).
Tarantino, Tinevez, Crowell, Boisson, Henriques, Mhlanga, Agou, Israël, Laplantine: "TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures." in: The Journal of cell biology, Vol. 204, Issue 2, pp. 231-45, (2014) (PubMed).
Boisson, Laplantine, Prando, Giliani, Israelsson, Xu, Abhyankar, Israël, Trevejo-Nunez, Bogunovic, Cepika, MacDuff, Chrabieh, Hubeau, Bajolle, Debré, Mazzolari, Vairo, Agou, Virgin, Bossuyt, Rambaud et al.: "Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency. ..." in: Nature immunology, Vol. 13, Issue 12, pp. 1178-86, (2012) (PubMed).
Fan, Meng, Laterra, Rosen: "Role of Src signal transduction pathways in scatter factor-mediated cellular protection." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7561-77, (2009) (PubMed).
Lobry, Lopez, Israël, Weil: "Negative feedback loop in T cell activation through IkappaB kinase-induced phosphorylation and degradation of Bcl10." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 3, pp. 908-13, (2007) (PubMed).
Panopoulos, Harraz, Engelhardt, Zandi et al.: "Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB ..." in: The Journal of biological chemistry, Vol. 280, Issue 4, pp. 2912-23, (2005) (PubMed).
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).