ISG15 antibody (N-Term)

Catalog No. ABIN387983

The Rabbit Polyclonal anti-ISG15 antibody has been validated for WB and IHC (p). It is suitable to detect ISG15 in samples from Human. There are 5+ publications available.

Quick Overview for ISG15 antibody (N-Term) (ABIN387983)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB02791

Product Details anti-ISG15 Antibody

Binding Specificity: AA 31-61, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ISG15 antibody (ABIN387983)

Cheriyamundath, Basu, Haase, Doernberg, Gavert, Brabletz, Ben-Zeev: "ISG15 induction is required during L1-mediated colon cancer progression and metastasis." in: Oncotarget, Vol. 10, Issue 67, pp. 7122-7131, (2019) (PubMed).

Speer, Li, Buta, Payelle-Brogard, Qian, Vigant, Rubino, Gardner, Wedeking, Hermann, Duehr, Sanal, Tezcan, Mansouri, Tabarsi, Mansouri, Francois-Newton, Daussy, Rodriguez, Lenschow, Freiberg et al.: "ISG15 deficiency and increased viral resistance in humans but not mice. ..." in: Nature communications, Vol. 7, pp. 11496, (2018) (PubMed).

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, (2016) (PubMed).

Huang, Bulavin: "Oncogene-mediated regulation of p53 ISGylation and functions." in: Oncotarget, Vol. 5, Issue 14, pp. 5808-18, (2015) (PubMed).

Schanz, Baston-Büst, Heiss, Beyer, Krüssel, Hess: "Interferon stimulated gene 15 expression at the human embryo-maternal interface." in: Archives of gynecology and obstetrics, Vol. 290, Issue 4, pp. 783-9, (2014) (PubMed).

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15

Background: ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

Molecular Weight: 17888

Gene ID: 9636

NCBI Accession: NP_005092

UniProt: P05161