PRDM14 antibody (N-Term)

Catalog No. ABIN2845429

This Rabbit Polyclonal antibody specifically detects PRDM14 in WB, IF and IHC (p). It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for PRDM14 antibody (N-Term) (ABIN2845429)

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1405

Product Details anti-PRDM14 Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PRDM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRDM14.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PRDM14 antibody (ABIN2845429)

Sugawa, Araúzo-Bravo, Yoon, Kim, Aramaki, Wu, Stehling, Psathaki, Hübner, Schöler: "Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile." in: The EMBO journal, Vol. 34, Issue 8, pp. 1009-24, (2015) (PubMed).

Tsuneyoshi, Sumi, Onda, Nojima, Nakatsuji, Suemori: "PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells." in: Biochemical and biophysical research communications, Vol. 367, Issue 4, pp. 899-905, (2008) (PubMed).

Watanabe, Toyota, Kondo, Suzuki, Imai, Ohe-Toyota, Maruyama, Nojima, Sasaki, Sekido, Hiratsuka, Shinomura, Imai, Itoh, Tokino: "PRDM5 identified as a target of epigenetic silencing in colorectal and gastric cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 16, pp. 4786-94, (2007) (PubMed).

Nishikawa, Toyota, Suzuki, Honma, Fujikane, Ohmura, Nishidate, Ohe-Toyota, Maruyama, Sonoda, Sasaki, Urano, Imai, Hirata, Tokino: "Gene amplification and overexpression of PRDM14 in breast cancers." in: Cancer research, Vol. 67, Issue 20, pp. 9649-57, (2007) (PubMed).

Target Details for PRDM14

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Alternative Name: PRDM14 (PFM11)

Background: Similar to acetylation and phosphorylation, histone methylation at the N terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET domain family, and another 17 make up the PR domain family that is related to the SET domain family. PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor.

Molecular Weight: 64062

Gene ID: 63978

NCBI Accession: NP_078780

UniProt: Q9GZV8