Ubiquitin antibody (AA 36-66)

Catalog No. ABIN388040

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin (Ub)

Binding Specificity: AA 36-66

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Y, B, E, D, Pr, M, Pig, Rat, Sh, C, Hs, Ha, X, Rb

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin.

Clone: RB2702

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Ubiquitin antibody (ABIN388040)

Huang, Wang, Wright, Yang, Zhou, Li, Yang, Small, Parmacek: "Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 14, pp. 4447-52, (2015) (PubMed).

Vernes, Nicod, Elahi, Coventry, Kenny, Coupe, Bird, Davies, Fisher: "Functional genetic analysis of mutations implicated in a human speech and language disorder." in: Human molecular genetics, Vol. 15, Issue 21, pp. 3154-67, (2006) (PubMed).

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin (Ub Products)

Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Molecular Weight: 77039

Gene ID: 7314

UniProt: P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway