ATG7 antibody (AA 284-313)

Catalog No. ABIN388521

This anti-ATG7 antibody is a Rabbit Polyclonal antibody detecting ATG7 in WB and IHC (p). Suitable for Human and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for ATG7 antibody (AA 284-313) (ABIN388521)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7469

Product Details anti-ATG7 Antibody

Binding Specificity: AA 284-313

Predicted Reactivity: C, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-313 amino acids from the Central region of human ATG7.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG7 antibody (ABIN388521)

Ma, Molusky, Song, Hu, Fang, Rui, Mathew, Pennathur, Liu, Cheng, Guan, Lin: "Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 10, pp. 1643-54, (2013) (PubMed).

Ma, Panda, Lin: "Temporal orchestration of circadian autophagy rhythm by C/EBP?." in: The EMBO journal, Vol. 30, Issue 22, pp. 4642-51, (2012) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molecular Weight: 77960

Gene ID: 10533

NCBI Accession: NP_001129503, NP_001138384, NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy