ATG16L1 antibody (AA 161-190)

Catalog No. ABIN388542

This anti-ATG16L1 antibody is a Rabbit Polyclonal antibody detecting ATG16L1 in WB, IF and IHC (p). Suitable for Human and Mouse. This Primary Antibody has been cited in 8+ publications.

Quick Overview for ATG16L1 antibody (AA 161-190) (ABIN388542)

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG16L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB07490

Product Details anti-ATG16L1 Antibody

Binding Specificity: AA 161-190

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG16L1 antibody (ABIN388542)

Li, Guo, Yang, Li, Yang, Liu, Liu, Jiang: "1,25(OH)2 D3 attenuates hepatic steatosis by inducing autophagy in mice." in: Obesity (Silver Spring, Md.), Vol. 25, Issue 3, pp. 561-571, (2017) (PubMed).

Wang, Chen, Yeo, Karsli-Uzunbas, White, Mizushima, Virgin, Guan: "Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide." in: The Journal of cell biology, Vol. 212, Issue 5, pp. 545-60, (2016) (PubMed).

Zhang, Li, Zhang, Zhang, Teves, Liu, Strauss, Pazour, Foster, Hess, Zhang: "Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice." in: Molecular biology of the cell, (2016) (PubMed).

Wu, Zhang, Lu, Xia, Zhou, Petrof, Claud, Chen, Chang, Carmeliet, Sun: "Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis." in: Gut, Vol. 64, Issue 7, pp. 1082-94, (2015) (PubMed).

Wang, Mendonsa, Symington, Zhang, Cadwell, Virgin, Mysorekar: "Atg16L1 deficiency confers protection from uropathogenic Escherichia coli infection in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. 11008-13, (2012) (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, (2011) (PubMed).

Travassos, Carneiro, Ramjeet, Hussey, Kim, Magalhães, Yuan, Soares, Chea, Le Bourhis, Boneca, Allaoui, Jones, Nuñez, Girardin, Philpott: "Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry." in: Nature immunology, Vol. 11, Issue 1, pp. 55-62, (2009) (PubMed).

Nomura, Uzawa, Yamano, Fushimi, Ishigami, Kouzu, Koike, Siiba, Bukawa, Yokoe, Kubosawa, Tanzawa: "Overexpression and altered subcellular localization of autophagy-related 16-like 1 in human oral squamous-cell carcinoma: correlation with lymphovascular invasion and lymph-node metastasis." in: Human pathology, Vol. 40, Issue 1, pp. 83-91, (2008) (PubMed).

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: ATG16L

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Molecular Weight: 68265

Gene ID: 55054

NCBI Accession: NP_001177195, NP_001177196, NP_060444, NP_110430, NP_942593

UniProt: Q676U5

Pathways: Autophagy