DDX58 antibody (C-Term)

Catalog No. ABIN388602

This anti-DDX58 antibody is a Rabbit Polyclonal antibody detecting DDX58 in WB. Suitable for Human and Mouse. This Primary Antibody has been cited in 9+ publications.

Quick Overview for DDX58 antibody (C-Term) (ABIN388602)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB8008

Product Details anti-DDX58 Antibody

Binding Specificity: AA 894-925, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This RIG-I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 894-925 amino acids from the C-terminal region of human RIG-I.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DDX58 antibody (ABIN388602)

Munday, Howell, Barr, Hiscox: "Proteomic analysis of mitochondria in respiratory epithelial cells infected with human respiratory syncytial virus and functional implications for virus and cell biology." in: The Journal of pharmacy and pharmacology, Vol. 67, Issue 3, pp. 300-18, (2015) (PubMed).

Huang, Kolokoltsova, Yun, Seregin, Ronca, Koma, Paessler: "Highly Pathogenic New World and Old World Human Arenaviruses Induce Distinct Interferon Responses in Human Cells." in: Journal of virology, Vol. 89, Issue 14, pp. 7079-88, (2015) (PubMed).

Crill, Furr-Rogers, Marriott: "RIG-I is required for VSV-induced cytokine production by murine glia and acts in combination with DAI to initiate responses to HSV-1." in: Glia, (2015) (PubMed).

Feng, Langereis, Lork, Nguyen, Hato, Lanke, Emdad, Bhoopathi, Fisher, Lloyd, van Kuppeveld: "Enterovirus 2Apro targets MDA5 and MAVS in infected cells." in: Journal of virology, Vol. 88, Issue 6, pp. 3369-78, (2014) (PubMed).

Huang, Kolokoltsova, Yun, Seregin, Poussard, Walker, Brasier, Zhao, Tian, de la Torre, Paessler: "Junín virus infection activates the type I interferon pathway in a RIG-I-dependent manner." in: PLoS neglected tropical diseases, Vol. 6, Issue 5, pp. e1659, (2012) (PubMed).

Furr, Moerdyk-Schauwecker, Grdzelishvili, Marriott: "RIG-I mediates nonsegmented negative-sense RNA virus-induced inflammatory immune responses of primary human astrocytes." in: Glia, Vol. 58, Issue 13, pp. 1620-9, (2010) (PubMed).

Barral, Sarkar, Fisher, Racaniello: "RIG-I is cleaved during picornavirus infection." in: Virology, Vol. 391, Issue 2, pp. 171-6, (2009) (PubMed).

Bao, Liu, Shan, Li, Garofalo, Casola: "Human metapneumovirus glycoprotein G inhibits innate immune responses." in: PLoS pathogens, Vol. 4, Issue 5, pp. e1000077, (2008) (PubMed).

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, (2008) (PubMed).

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: RIG-I

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, RIG-I triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Molecular Weight: 106600

Gene ID: 23586

NCBI Accession: NP_055129

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C