SMURF1 antibody (N-Term)

Catalog No. ABIN388828

This Rabbit Polyclonal antibody specifically detects SMURF1 in WB and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 4+ publications.

Quick Overview for SMURF1 antibody (N-Term) (ABIN388828)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB04101

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 66-96, N-Term

Predicted Reactivity: X

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SMURF1 antibody (ABIN388828)

He, Yang, Isales, Shi: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-? inhibition of mesenchymal stem cell osteogenic differentiation." in: PLoS ONE, Vol. 7, Issue 3, pp. e31717, (2012) (PubMed).

Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).

Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).

Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background: Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Molecular Weight: 86114

Gene ID: 57154

NCBI Accession: NP_001186776, NP_065162, NP_851994

UniProt: Q9HCE7

Pathways: Regulation of Cell Size