DAAM1 antibody (C-Term)

Catalog No. ABIN389180

The Rabbit Polyclonal anti-DAAM1 antibody has been validated for WB and IHC (p). It is suitable to detect DAAM1 in samples from Human. There is 1 publication available.

Quick Overview for DAAM1 antibody (C-Term) (ABIN389180)

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB10612

Product Details anti-DAAM1 Antibody

Binding Specificity: AA 1034-1063, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1034-1063 amino acids from the C-terminal region of human DAAM1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DAAM1 antibody (ABIN389180)

Nishimura, Ito, Saito, Hiver, Shigetomi, Ikenouchi, Takeichi: "DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility." in: The Journal of cell biology, Vol. 215, Issue 4, pp. 559-573, (2017) (PubMed).

Target Details for DAAM1

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Alternative Name: DAAM1

Background: Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.

Molecular Weight: 123473

Gene ID: 23002

NCBI Accession: NP_001257449, NP_055807

UniProt: Q9Y4D1

Pathways: WNT Signaling