ERK1/2 antibody (pThr202, pTyr204)

Catalog No. ABIN389990

The Rabbit Polyclonal anti-ERK1/2 antibody has been validated for WB and DB. It is suitable to detect ERK1/2 in samples from Human. There is 1 publication available.

Quick Overview for ERK1/2 antibody (pThr202, pTyr204) (ABIN389990)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Clone: RB15981

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr202, pTyr204

Predicted Reactivity: D, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ERK1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ERK1/2 antibody (ABIN389990)

Hu, Zhou, Zhao, Wu: "Integrin α6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy." in: Scientific reports, Vol. 6, pp. 33376, (2018) (PubMed).

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK1/2

Background: MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.

Molecular Weight: 43136

Gene ID: 5595

NCBI Accession: NP_001035145, NP_001103361, NP_002737

UniProt: P27361