WISP1 antibody (AA 171-200)

Catalog No. ABIN390187

This Rabbit Polyclonal antibody specifically detects WISP1 in WB and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for WISP1 antibody (AA 171-200) (ABIN390187)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB2115-2116

Product Details anti-WISP1 Antibody

Binding Specificity: AA 171-200

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human WISP1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-WISP1 antibody (ABIN390187)

Ji, Jia, Jia, Ji, Hargest, Jiang: "WISP-2 in human gastric cancer and its potential metastatic suppressor role in gastric cancer cells mediated by JNK and PLC-? pathways." in: British journal of cancer, Vol. 113, Issue 6, pp. 921-33, (2015) (PubMed).

Target Details for WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP1

Background: Wisp1 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. Wisp1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level.

Molecular Weight: 40331

Gene ID: 8840

NCBI Accession: NP_001191798, NP_001191799, NP_003873, NP_543028

UniProt: O95388

Pathways: WNT Signaling, Growth Factor Binding