Sclerostin antibody (N-Term)

Catalog No. ABIN390193

This Rabbit Polyclonal antibody specifically detects Sclerostin in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for Sclerostin antibody (N-Term) (ABIN390193)

Target: Sclerostin (SOST)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sclerostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB12388

Product Details anti-Sclerostin Antibody

Binding Specificity: AA 12-42, N-Term

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-Sclerostin antibody (ABIN390193)

Zarei, Hulley, Sabokbar, Javaid: "Co-expression of DKK-1 and Sclerostin in Subchondral Bone of the Proximal Femoral Heads from Osteoarthritic Hips." in: Calcified tissue international, Vol. 100, Issue 6, pp. 609-618, (2018) (PubMed).

Lin, Lin, Chang, Wang, Lai: "Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation." in: Bioelectromagnetics, Vol. 36, Issue 7, pp. 494-505, (2015) (PubMed).

Target Details for Sclerostin

Target: Sclerostin (SOST)

Alternative Name: SOST

Background: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Molecular Weight: 24031

Gene ID: 50964

NCBI Accession: NP_079513

UniProt: Q9BQB4