LATS1 antibody (N-Term)

Catalog No. ABIN391033

This Rabbit Polyclonal antibody specifically detects LATS1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for LATS1 antibody (N-Term) (ABIN391033)

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LATS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3029

Product Details anti-LATS1 Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LATS1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-LATS1 antibody (ABIN391033)

Eiring, Neviani, Santhanam, Oaks, Chang, Notari, Willis, Gambacorti-Passerini, Volinia, Marcucci, Caligiuri, Leone, Perrotti: "Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis." in: Blood, Vol. 111, Issue 2, pp. 816-28, (2008) (PubMed).

Target Details for LATS1

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Alternative Name: LATS1

Background: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Molecular Weight: 126870

Gene ID: 9113

NCBI Accession: NP_001257448, NP_004681

UniProt: O95835

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location