EN1 antibody (N-Term)

Catalog No. ABIN391430

The Rabbit Polyclonal anti-EN1 antibody has been validated for WB, IHC (p) and IF. It is suitable to detect EN1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for EN1 antibody (N-Term) (ABIN391430)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: RB13885

Product Details anti-EN1 Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. IF: 1:25. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-EN1 antibody (ABIN391430)

Majumdar, Kanafi, Bhonde, Gupta, Datta: "Differential Neuronal Plasticity of Dental Pulp Stem Cells From Exfoliated Deciduous and Permanent Teeth Towards Dopaminergic Neurons." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 2048-63, (2017) (PubMed).

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1 (Engrailed 1)

Background: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Molecular Weight: 40115

Gene ID: 2019

NCBI Accession: NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis