CCL5 antibody

Catalog No. ABIN400758

This anti-CCL5 antibody is a Mouse Monoclonal antibody detecting CCL5 in Neut, EIA and IHC (p). Suitable for Human.

Quick Overview for CCL5 antibody (ABIN400758)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Neutralization (Neut), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8-PP-11

Product Details anti-CCL5 Antibody

Specificity: Recognizes Human RANTES. The antibody effectively inhibits Chemotaxis of Human Monocytes, Human Lymphocytes and Rat peritoneal Macrophages induced by Human RANTES.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: C-C motif chemokine 5, D17S136E, SCYA5, Small-inducible cytokine A5, SIS-delta, EoCP,T-cell-specific protein RANTES, cell-specific protein P228, Eosinophil-chemotactic cytokine

Purification: Protein A Chromatography.

Immunogen: RANTES

Isotype: IgG2b

Application Details

Application Notes: ELISA: 2-4 μg/mL. Neutralization. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This CCL5 antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) Human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 40 % Glycerol without preservatives.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration