CD28 antibody

Catalog No. ABIN400890

This anti-CD28 antibody is a Mouse Monoclonal antibody detecting CD28 in FACS, WB, IF, IP, Func and IHC (fro). Suitable for Human and Primate.

Quick Overview for CD28 antibody (ABIN400890)

Target: CD28

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CD28-2

Product Details anti-CD28 Antibody

Specificity: The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Purification: Affinity Chromatography on Protein A

Sterility: 0.2 μm filtered

Immunogen: DC28. 1. 3. 3 hybridoma cells

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western blot. Immunohistochemistry on frozen sections. Immunoflourescence. Functional Application. T cell costimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilization. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response