CD28 antibody (FITC)

Catalog No. ABIN400892

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS. It is suitable to detect CD28 in samples from Human and Primate.

Quick Overview for CD28 antibody (FITC) (ABIN400892)

Target: CD28

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: CD28-2

Product Details anti-CD28 Antibody (FITC)

Specificity: The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Primates

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Immunogen: DC28. 1. 3. 3 hybridoma cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response